1998

Colaboración con el Laboratorio de Microbiología del doctor Alexander Tomasz en Rockefeller University en Nueva York

Collaboration with the Microbiology Laboratory of Dr. Alexander Tomasz at the Rockefeller University in Nueva York

El propósito de la colaboración fue la caracterización molecular de los aislamientos de Streptococcus pneumoniae resistentes a la penicilina inicialmente con el empleo de la técnica de electroforesis de campo pulsado (PFGE) y posteriormente con la secuenciación (Multi locus sequencing typing, MLST) para determinar los mecanismos de propagación y posible origen de las cepas resistentes en América Latina.

Las técnicas han sido ampliamente usadas en la región y han permitido establecer la diversidad genética de los aislamientos y la expansión de clones internacionales y de clones menores en los países estudiados. Un par de ejemplos son los estudios del clon Colombia5 ST289 y su presencia en los países latinoamericanos y más recientemente los estudios de los aislamentos del serotipo 19A.

The purpose of the collaboration was the molecular characterization of the penicillin-resistant Streptococcus pneumoniae isolates, initially using pulse field gel electrophoresis (PFGE) and later on, sequencing (Multi locus sequencing typing, MLST)  to obtain some insights into the mechanisms of spread and possible origin of the resistant strains in Latin America.

These techniques have been widely used in the Region and have allowed to establish the genetic diversity of the isolates and the expansion of international clones and spread of minor clones in countries where studies were conducted. Two examples are, the study  of clon Colombia5 ST289, and its presence in Latin American countries and more recently the studies of serotype 19A isolates.

Publicaciones/Publications

 

  • Tomasz A, Corso A, Severina EP, Echániz-Aviles G, Brandileone MC, Camou T, Castañeda E, Figueroa O, Rossi A, Di Fabio JL. Molecular epidemiologic characterization of penicillin-resistant Streptococcus pneumoniae invasive pediatric isolates recovered in six Latin-American countries: an overview. Microb Drug Resist. 1998;4:195-207.
  • De Cunto Brandileone M.C., Di Fabio J.L., Vieira V.S.D., Zanella R.C., Casagrande S.T., Pignatari A.C., Tomasz A. Geographic distribution of penicillin resistance of Streptococcus pneumoniae in Brazil: Genetic relatedness. Microb Drug Resist. 1998;4:209-17.
  • Camou T., Hortal M., Tomasz A. The apparent importation of penicillin-resistant capsular type 14 Spanish/French clone of Streptococcus pneumoniae into Uruguay in the early 1990s. Microb Drug Resist. 1998;4:219-224.
  • Rossi A., Corso A., Pace J., Regueira M., Tomasz A. Penicillin-resistant Streptococcus pneumoniae in Argentina: Frequent occurrence of an internationally spread serotype 14 clone. Microb Drug Resist. 1998;4:225-231.
  • Castañeda E., Peñuela I., Vela M.C., Tomasz A. Penicillin-resistant Streptococcus pneumoniae in Colombia: Presence of international epidemic clones. Microb Drug Resist. 1998;4:233-239.
  • Echániz-Aviles G., Velázquez-Meza M.E., Carnalla-Barajas M.N., Soto-Noguerón A., Di Fabio J.L., Solórzano-Santos F., Jiménez-Tapia Y., Tomasz A. Predominance of the multiresistant 23F international clone of Streptococcus pneumoniae among isolates from Mexico. Microb Drug Resist. 1998;4:241-246.
  • Tamayo M, Sá-Leâo R, Santos Sanches I, Castañeda E, De Lencastre H. Dissemination of chloramphenicol and tetracycline-resistant but penicillin susceptible invasive clone of serotype 5 Streptococcus pneumoniae in Colombia. J Clin Microbiol 1999;37:2337-42.
  • Gamboa L, Camou T, Hortal M, Castañeda E, Sireva-Vigía Working Group. Dissemination of Streptococcus pneumoniae clone Colombia5 in 19 in Latin America. J Clin Microbiol 2002;40:3942-50.
  • Zemlicková H, Crisóstomo MI, Brandileone MC, Camou T, Castañeda E, Corso A, Echániz-Aviles G, Pásztor M, Tomasz A. Serotypes and clonal types of penicillin-susceptible Streptococcus pneumoniae causing invasive disease in children in five Latin American countries. Microb Drug Resist. 2005;11:195-204.
  • Pneumococcal Molecular Epidemiology Network (PMEN). Fecha de consulta: 10 de septiembre de 2010. Disponible en: www.pneumogen.net/pmen
  • Gagetti P, Faccone D, Reijtman V, Fossati S, Rodriguez M, Veliz O, et al. Characterization of Streptococcus pneumoniae invasive serotype 19A isolates from Argentina (1993-2014). Vaccine 2017;35(35 Pt B):4548–53.
  • Moreno J, Duarte C, Cassiolato AP,Chanto Chacón G, Alarcón P, Sánchez J, Narváez San Martin Y, Valenzuela C, et al. Molecular characterization of Latin American invasive Streptococcus pneumoniae serotype 19A isolates. Vaccine. 2020;38(19):3524-3530. doi:10.1016/j.vaccine.2020.03.030